The SIF provides isotope analysis (
Analysis of
Trace gas isotope ratios are measured using a SerCon Cryoprep trace gas concentration system interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). Gas samples are purged from vials through a double-needle sampler into a helium carrier stream
As
Final delta
Analysis |
Instrument |
Turnaround Time (weeks) |
Price per Sample (USD) |
PDZ Europa 20-20, |
4 |
$9.50 |
|
PDZ Europa 20-20, |
4 |
$9.50 |
|
PDZ Europa 20-20, Cryoprep | 4 |
$9.50 |
|
| 15N Analysis of NO3 in water using Pseudomonas chlororaphis bacteria. Samples prepared by the SIF | PDZ Europa 20-20, | 7-8 |
$20.00 |
| Nitrogen and 18O Analysis of NO3 in water using Pseudomonas aureofaciens bacteria. Samples prepared by the SIF | PDZ Europa 20-20, | 7-8 |
$20.00 |
*Please contact Emily to schedule preparation of the denitrifying bacteria.
Gas samples are supplied in septum capped vials preferably at atmospheric pressure
Gas samples are collected in
For NO3 in water preparation, please see the denitrifier articles in the Reference section. Water samples requiring preparation by the SIF should be filtered and shipped frozen in 30ml-60ml Nalgene-type bottles. Samples requiring extensive handling by the SIF (ie. filtering) will be charged an additional $1.00 handling fee per sample. Please provide a minimum of 10mLs of each water sample.
Carefully package groups of exetainers in ziplock bags, small boxes, or their original boxes. Then package these sets in larger, sturdy boxes with styrofoam peanuts or bubble wrap. Make sure the samples are very secure, loose vials can shatter during shipping. These gas samples do not require refrigeration during shipping.
Water samples for bacteria denitrification preparation should be shipped frozen and packaged in an insulated box with dry ice or blue ice blocks. Coolers will not be returned.
*NEW* Please complete an Analysis Order Form and Sample List for your samples. E-mail the completed forms to sif@ucdavis.edu and include a printed copy with your samples. The SIF uses this form to track your samples and to contact you regarding receipt of samples, data, and invoicing. For mixed analysis requests, we recommend completing a separate form for each type of analysis.
Contact information
UC Davis Stable Isotope Facility
Department of Plant Sciences
One Shields Avenue, Mail Stop 1
Davis, CA 95616
USA
Phone:(530) 754-7517, Fax: (530) 752-4361
E-mail: sif@ucdavis.edu
| Manufacturer / Part# |
Description |
Unit |
Labco / 839W, 739W |
Exetainers: 12mL Soda Glass Vials, flat bottomed, labeled, white cap, evacuated (839W) or non-evacuated (739W) |
Case of 1000* |
Grace / 98788 |
Vials For |
Pack of 100 |
Grace/ 98683 |
Crimp caps for Headspace Vials: 20mm Aluminum Seal with Black Butyl Liner |
Pack of 100 |
*Note: Labco has a new vial style that ends in ###W/W. We DO NOT recommend these vials when sending samples to the SIF because the vials are too wide for our autosampler rack. Supplier: Labco UK + International or Labco USA + Canada
References for NO3 in Water
15N Analysis of NO3 in water using Pseudomonas chlororaphis bacteria:
D. M. Sigman, K. L. Casciotti, M. Andreani, C. Barford, M. Galanter, and
J. K. Böhlke. A Bacterial Method for the Nitrogen Isotopic Analysis of
Nitrate in Seawater and Freshwater. Anal. Chem., 73 (17), 4145 -4153, 2001.
1) Avoid over-tightening exetainer caps. Vials will leak when caps are over-tightened, causing the septa to pucker and pull away from the vial. Turn caps until snug, but not so far that the septa start to pucker.
2) Do not apply silicone grease, adhesives, or sealant to the tops of the vials. These materials clog the autosampler needle. The process of removing these sealants increases the risk of losing your gas sample due to dislodged septum.
Updated June 29, 2009