Compound-Specific
Stable Isotope
Analysis


Analysis

Pricing

Sample Preparation

Tips

Sample Submission




















UC Davis Plant Sciences


n-Alkane Sample Preparation


Submit samples in standard GC vials (volume: 2 mL O.D. x length: 12 x 32 mm) with screw caps (9-425 or 10-425). Use inserts for low-concentration samples. With inserts, we can inject up to 4 µL from as little as 30 µL solvent. Use vials with a write-on patch or use clear tape and permanent marker to label vials. Do not wrap vials with opaque marking tape – this makes it difficult to view liquid level. Thick tape causes vials to stick in the autosampler tray.

Total concentration of n-alkanes in samples should range from 0.5 to 1 micrograms C per microliter, for typical samples having 10-20 moderate-sized peaks. Lower total concentration (~0.5 micrograms C per microliter) are acceptable for samples having ≤10 major peaks. Large n-alkane peaks are 50-140 ng C and the smallest detectable peaks are ~1.5 ng C.

Example chromatogram








 
e-mail: sif@ucdavis.edu | phone: 530-752-8100 | fax: 530-752-4361
UC Davis Stable Isotope Facility | Department of Plant Sciences
One Shields Avenue | Davis, California, 95616 | USA